TECHNICAL SPECIFICATIONS
- Ready to use reagent strip format
- 15 minute test
- Up to 8 tests simultaneously
- 4 individual chambers, 5+1+1+1 detection channels
- Convenient 24 tests pack size
- Excellent calibration stability
- Range or specialized parameters
Chemiluminescence immunoassay (CLIA) is a highly sensitive and specific diagnostic technique used in clinical laboratories to detect a wide range of biomolecules, including hormones, proteins, antibodies, antigens, and drugs. This advanced diagnostic tool has gained popularity due to its enhanced sensitivity, broad dynamic range, and relatively short assay time. In this article, we delve into the principles, applications, advantages, and future prospects of chemiluminescence immunoassays in the realm of diagnostics.
Chemiluminescence is the emission of light as a result of a chemical reaction. In a CLIA, this reaction involves the interaction between a labeled molecule, typically an enzyme or a fluorophore, and a target biomolecule. The labeled molecule is conjugated to an antibody or antigen, forming a complex. When the labeled molecule comes into contact with its corresponding biomolecule, a chemical reaction is initiated, resulting in the emission of light. The intensity of the emitted light is directly proportional to the amount of the target biomolecule present in the sample.
The chemiluminescent reaction is catalyzed by specific enzymes such as alkaline phosphatase, horseradish peroxidase, or firefly luciferase. The substrate for the enzyme reacts with it, producing an excited-state intermediate that, upon returning to the ground state, emits light. This light emission is detected and measured to quantify the target biomolecule in the sample.
Rapid Chemiluminescence Immunoassay